Mark the glass plate 1 cm below the comb teeth. ![]() Place a comb completely into the assembled gel cassette. Assembling the Mini-PROTEAN casting stand and frame. Orient the spacer plate so that the labeling is up.į. Select a spacer plate of the desired gel thickness and place a short plate on top of it (see Figure 3a). Place the casting frame upright with the pressure cams in the open position and facing forward on a flat surface. Glass Cassette and Casting Stand Assembly Note: All glass plates should be clean and dry. Section 2 Setup and Basic Operation 2.1 Gel Cassette Preparation Handcast Gels 1. The Mini-PROTEAN are not compatible with repeated exposure to 100% TEMED. Call 1-800-4-BIORAD (US) or your local Bio-Rad representative for technical information regarding chemical compatibility of the Mini-PROTEAN Tetra cell with various laboratory reagents. Use of organic solvents voids all warranties. 2.ġ.3 Specifications Casting Stand* Polycarbonate Pin, retaining ring, and spring Casting Frames* Stainless steel Polysulfone Gray gaskets Thermoplastic rubber (gray) Electrode Assembly Glass-filled polybutylene terephthalate Electrodes Platinum wire, 0.Ĭhemical Compatibility Mini-PROTEAN Tetra cell components are not compatible with acetone or ethanol. Lid Banana plug jacks Notch on U-shaped gasket Gel cassette Electrode assembly Mini tank Fig. It holds the gel sandwich and houses the sealing gasket. Companion Assembly The companion assembly allows you to run gels 3 and 4. The anode (lower electrode) banana plug is identified with a red marker and the cathode (upper electrode) banana plug with a black marker. It houses the sealing gasket, the upper and lower electrodes, and the connecting banana plugs. ![]() 1.Įlectrode Assembly The electrode assembly holds the gel sandwich. The cell can run one or four gels, and the mini tank is compatible with other Bio-Rad electrode modules for tank blotting, 2-D electrophoresis, and electroelution. The Mini-PROTEAN Tetra system includes a casting stand and glass plates with permanently bonded gel spacers that simplify handcasting and eliminate leaking during casting. Section 1 General Information 1.1 Introduction The Mini-PROTEAN® Tetra cell runs both handcast gels and Ready Gel® precast gels interchangeably. Table of Contents Section 1 General Information 1.1 Introduction 1.2 Components 1.3 Specifications 1.4 Safety 1 1 1 4 5 Section 2 Setup and Basic Operation 2.1 Gel Cassette Preparation 2.2 Electophoresis Module Assembly and Sample Loading 6 6 9 Section 3 Separation Theory and Optimization 3.1 Introduction 3.2 SDS-PAGE (Laemmli) Buffer System 3.3 Native PAGE 15 15 16 17 Section 4 Reagent Preparation and Stock Solutions 4.1 Volumes Required per Gel 4.2 SDS-PAGE (Laemmli) Buffer System 4. Laemmli-like separation in the presence of SDS. ![]() Can be used for denaturing electrophoresis with standard running buffer containing SDS.There is a wide range of Mini-PROTEAN TGX Precast Protein Gels, including other gels with 10 wells, in both single percentages and gradients. Run up to 4 Mini-PROTEAN TGX Precast Protein Gels in 35 min using the Mini-PROTEAN Tetra Cell. Long-life TGX (Tris-Glycine eXtended) Gels have a novel formulation and can be used for both standard denaturing protein separations as well as native electrophoresis. Use these Mini-PROTEAN TGX Precast Protein Gels for separation of polypeptides ~2–400 kD.
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